Live imaging of the lung
T Brzoska, TW Kaminski… - Current protocols in …, 2020 - Wiley Online Library
T Brzoska, TW Kaminski, MF Bennewitz, P Sundd
Current protocols in cytometry, 2020•Wiley Online LibraryLive imaging is critical to determining the dynamics and spatial interactions of cells within
the tissue environment. In the lung, this has proven to be difficult due to the motion brought
about by ventilation and cardiac contractions. A previous version of this Current Protocols in
Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse
lung. Here, we update those protocols by adding new methodologies, new approaches for
quantitative image analysis, and new areas of potential application.© 2020 Wiley …
the tissue environment. In the lung, this has proven to be difficult due to the motion brought
about by ventilation and cardiac contractions. A previous version of this Current Protocols in
Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse
lung. Here, we update those protocols by adding new methodologies, new approaches for
quantitative image analysis, and new areas of potential application.© 2020 Wiley …
Abstract
Live imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion brought about by ventilation and cardiac contractions. A previous version of this Current Protocols in Cytometry article reported protocols for imaging ex vivo live lung slices and the intact mouse lung. Here, we update those protocols by adding new methodologies, new approaches for quantitative image analysis, and new areas of potential application. © 2020 Wiley Periodicals LLC.
Basic Protocol 1: Live imaging of lung slices
Support Protocol 1: Staining lung sections with fluorescent antibodies
Basic Protocol 2: Live imaging in the mouse lung
Support Protocol 2: Intratracheal instillations
Support Protocol 3: Intravascular instillations
Support Protocol 4: Monitoring vital signs of the mouse during live lung imaging
Support Protocol 5: Antibodies
Support Protocol 6: Fluorescent reporter mice
Basic Protocol 3: Quantification of neutrophil‐platelet aggregation in pulmonary vasculature
Basic Protocol 4: Quantification of platelet‐dependent pulmonary thrombosis
Basic Protocol 5: Quantification of pulmonary vascular permeability
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